Design and optimization of a double-enzyme glucose assay in microfluidic lab-on-a-chip     

Yegermal Tesfaw Atalay  Daan Witters  Steven Vermeir  Nicolas Vergauwe  Pieter Verboven  Bart Nicola?  Jeroen Lammertyn 《Biomicrofluidics》2009,3(4)

An electrokinetic driven microfluidic lab-on-a-chip was developed for glucose quantification using double-enzyme assay. The enzymatic glucose assay involves the two-step oxidation of glucose, which was catalyzed by hexokinase and glucose-6-phosphate dehydrogenase, with the concomitant reduction of NADP⁺ to NADPH. A fluorescence microscopy setup was used to monitor the different processes (fluid flow and enzymatic reaction) in the microfluidic chip. A two-dimensional finite element model was applied to understand the different aspects of design and to improve the performance of the device without extensive prototyping. To our knowledge this is the first work to exploit numerical simulation for understanding a multisubstrate double-enzyme on-chip assay. The assay is very complex to implement in electrokinetically driven continuous system due to the involvement of many species, which has different transport velocity. With the help of numerical simulation, the design parameters, flow rate, enzyme concentration, and reactor length, were optimized. The results from the simulation were in close agreement with the experimental results. A linear relation exists for glucose concentrations from 0.01 to 0.10 g l⁻¹. The reaction time and the amount of enzymes required were drastically reduced compared to off-chip microplate analysis.  相似文献   

A double win: practice and participation in an action learning facilitators’ set     

Nicola Wilson 《Action Learning: Research and Practice》2019,16(2):178-186

This account of practice shares insights from past and present members of a self-facilitating action learning set, where each of the members is an action learning facilitator. Motivations for joining the set encompassed the opportunity to ‘practice what you preach’ by being a set member as well as a facilitator, the potential to refine and develop facilitation practices in a safe environment and space for personal development.  相似文献   

Users’ guides to the medical literature. Essentials of evidence‐based clinical practice     

Nicola Bexon 《Health information and libraries journal》2003,20(Z1):79-80

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Research and policy in education     

Nicola Ingram 《British Journal of Sociology of Education》2017,38(3):403-414

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Volunteers in Australian archives     

Annelie de Villiers  Nicola Laurent  Christopher Stueven 《档案与原稿》2017,45(2):118-135

Why do volunteers choose to contribute thousands of unpaid hours per week to Australian archives? This paper presents the results of a nationwide web survey that provides insight into the demographics, motivations and experiences of volunteers in Australian archives. The findings provide a representative overview of formal volunteers in Australian archives, determining ‘who’ they are, the value of the contributions they provide and the level of training and support offered to them. This study is a continuation of existing discussions about volunteers in Australian archives and represents an opportunity for the development of stronger relationships with Australian archival volunteers and, through them, the communities our archives serve.  相似文献   

Mentoring the Next Generation of Faculty: Supporting Academic Career Aspirations Among Doctoral Students     

Nicola Curtin  Janet Malley  Abigail J. Stewart 《Research in higher education》2016,57(6):714-738

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Self-reported impacts of volunteering in UK zoos and aquariums     

Charlotte Smith  Nicola Buckley  Elinor Bridges  Bethany Pavitt 《Cultural Trends》2018,27(1):18-32

ABSTRACT

Zoos and aquariums are popular visitor destinations, with around 30 million visits made to them annually throughout the UK and Ireland. The role and mission of modern zoos have evolved, with the conservation of world’s remaining biodiversity now being their major purpose. They seek to aid biodiversity through a combination of public education, in situ and ex situ conservation programmes, and applied scientific research. Directly relevant to this paper, they are also institutions that actively recruit, attract and utilise volunteers. However, the impacts of volunteering in zoos and aquariums, on the volunteers themselves, are under-researched. Here we show that, in a survey of more than 500 volunteers at 19 different zoological collections in the UK, zoo volunteers report positive impacts, specifically in relation to increases in their human and social capital. We also found that these benefits were more pronounced in younger volunteers, and with those volunteers who received more initial training. We conclude that while volunteering in UK zoos correlates with significant personal benefits to individuals, zoos could aim higher and should seek to do more to maximise the positive effects of connecting volunteers with the wildlife and nature found in zoo settings, as well as tailoring their volunteer training programmes for different age groups.  相似文献   

Effects of strength,explosive and plyometric training on energy cost of running in ultra-endurance athletes     

Nicola Giovanelli  Paolo Taboga  Enrico Rejc  Stefano Lazzer 《European Journal of Sport Science》2017,17(7):805-813

The aim of the present study was to evaluate the effects of a 12-week home-based strength, explosive and plyometric (SEP) training on the cost of running (Cr) in well-trained ultra-marathoners and to assess the main mechanical parameters affecting changes in Cr. Twenty-five male runners (38.2?±?7.1 years; body mass index: 23.0?±?1.1?kg·m^?2; V˙O₂max: 55.4?±?4.0 mlO₂·kg^?1·min^?1) were divided into an exercise (EG?=?13) and control group (CG?=?12). Before and after a 12-week SEP training, Cr, spring-mass model parameters at four speeds (8, 10, 12, 14?km·h^?1) were calculated and maximal muscle power (MMP) of the lower limbs was measured. In EG, Cr decreased significantly (p?<?.05) at all tested running speeds (?6.4?±?6.5% at 8?km·h^?1; ?3.5?±?5.3% at 10?km·h^?1; ?4.0?±?5.5% at 12?km·h^?1; ?3.2?±?4.5% at 14?km·h^?1), contact time (t_c) increased at 8, 10 and 12?km·h^?1 by mean +4.4?±?0.1% and t_a decreased by ?25.6?±?0.1% at 8?km·h^?1 (p?<?.05). Further, inverse relationships between changes in Cr and MMP at 10 (p?=?.013; r?=??0.67) and 12?km·h^?1 (p?<?.001; r?=??0.86) were shown. Conversely, no differences were detected in the CG in any of the studied parameters. Thus, 12-week SEP training programme lower the Cr in well-trained ultra-marathoners at submaximal speeds. Increased t_c and an inverse relationship between changes in Cr and changes in MMP could be in part explain the decreased Cr. Thus, adding at least three sessions per week of SEP exercises in the normal endurance-training programme may decrease the Cr.  相似文献   

Book Review: Blockbusters and Trade Wars: Popular Culture in a Globalized World     

Nicola Simpson 《媒体管理杂志》2013,15(1-2):139-140

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What is modelled during observational learning?     

Hodges NJ  Williams AM  Hayes SJ  Breslin G 《Journal of sports sciences》2007,25(5):531-545

In this article, we examine the question of what information is processed during observational learning by evaluating a variety of methods, theories, and empirical data. Initially, we review work involving neuroimaging techniques and infant imitation. We then evaluate data from behavioural experiments involving adults, wherein a variety of attempts have been made to isolate the critical or minimal information constraining the acquisition of coordination. This body of research has included comparisons between video and point-light displays, manipulations to the amount and type of information presented in the display, the collection of point-of-gaze data, and manipulations to the task context in terms of outcome goals. We conclude that observational learning is governed by specific features of the model's action (i.e. motions of the end effector) and the task (i.e. outcome constraints) and, in contrast with traditional theoretical modelling, more global aspects of a model (i.e. the relative motions within and between joints) do not appear to be the primary method for constraining action execution.  相似文献